Even if the MPGuino calibration is based on fill ups, basing it on several (or more) 10+ gallons fill ups is going going to give you much more accurate results than a some 100 mile runs.
Furthermore, even if the number is off, the relative numbers will be good for comparisons sake. So just go with it, I say. I didn't even calibrate mine, I just used a number from someone else and it's always been dead accurate.
*shrug*
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