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I'd go with practical experience. I've never gotten much over 50 mpg from any bike I've owned (most in the 650-850 CC range, but including a 350 CC Honda back in the '70s). I get over 70 mpg from the 1000 CC in my Insight.
For building a reverse trike, I'd think (on the basis of zero practical experience, of course) that it'd be easier to grab the engine, driveshafts, and all from a FWD car. Maybe look at some of the Locost designs, where there's some practical experience - on the front half, at least. Searching on "locost trike" brings up a bunch of hits.
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