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Definitely charge each battery individually so you know 100% that they are all fully charged. Then let them sit for a day to check resting voltages. If you have any that are connected in parallel (which it doesn't sound like you do), remember to separate them.
They should all be within 0.05V of each other at the end of the day. I would hazard a guess that battery number two will not be the same as the others, but you never know.
Even if they are all the same voltage, I have had it in the past where all batteries rest at the same voltage but one or two simply have next to no capacity once you start working them. I learned the hard way from using used batteries!
If battery two continues to crap out quickly after a full charge once you start working it, it's toast.
Good luck!
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