I don't know that staying in D at a light necessarily uses more fuel than N. After all, you haven't changed the throttle position, and the engine is turning at a lower RPM.
If you remove the spare, you might as well remove the jack and and lug wrench and save a few more lbs.
In my old Civic the back seat was pretty easy to remove, and the passenger seat isn't that hard either. Probably more effective than removing floor mats, and since you are getting in and out of your car all the time you might want the floor mats there to protect the carpet from the dirt being tracked in.
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If you look through the pages of this site you will find that the most mileage is gained from changing the way you drive. Aero, weight, induction, etc., mods won't make as much difference as slowing down and smoothing out your driving, but they will benefit you after you have changed your habits.
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